Villains

Pure Evil

 * Green Goblin (Norman Osborn)
 * Symbiote
 * Venom (Eddie Brock)

Criminals

 * Carjacker
 * Doctor Octopus (Otto Octavious)

Other

 * New Goblin (Harry Osborn)

List (In order of Appearance as a villain)

 * Carjacker
 * Green Goblin (Norman Osborn)
 * Doctor Octopus (Otto Octavious)
 * New Goblin (Harry Osborn)
 * Sandman (Flint Marko)
 * Venom (Eddie Brock)